Biotechnology - Proteomics: Principles and Techniques

Indian Institute of Technology Bombay

An introduction to proteomics: Basics of protein structure and function, An overview of systems biology, Evolution from protein chemistry to proteomics; Abundance-based proteomics: Sample preparation and prefractionation steps, Gel-based proteomics - two-dimensional gel electrophoresis (2-DE), two-dimensional fluorescence difference in-gel electrophoresis (DIGE), Staining techniques. Quantitative proteomics - stable isotope labeling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), isobaric tagging for relative and absolute quantitation (iTRAQ); Central role of mass spectrometry: ionization sources, mass analyzers, different types of mass spectrometers; Functional proteomics: Recombinational cloning, Interactomics - techniques to study protein-protein interactions, yeast two-hybrid, immunoprecipitation, protein microarrays, Nucleic Acid Programmable Protein Array (NAPPA), Label-free nanotechnologies in proteomics, Surface Plasmon Resonance (SPR); Modificomics: understanding post-translational modifications; Structural proteomics; Bioinformatics in proteomics; Challenges and future prospects of proteomics research.

Course Lectures